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Why my maker get no results?

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Why my maker get no results?

dcg@cau.edu.cn
Dear sir:
    I' have bben working on genome annotation these days.My process in as below:
    
    1. I split my contigs into 300 parts and deal with them simultaneously to speed up my process. 
    2. I used my splited-genome, protein, ESTs and RNA-seq to make the first alignment( est2genome=1,  AED_threshold=0.2 ). 
    3. Merge the maker.*_.master_datastore_index.log to get all the paths of results.
    4. gff_merge script to merge all the results in different dirs.

    However, there is no results returned. (My genome is about 3GB, but the gff of result is none.)
    index_all.log.all.gff    1KB
    index_all.log.all.maker.proteins.fasta    2837KB
    index_all.log.all.maker.transcripts.fasta    9866KB




    Where can the problems take place?
    Thanks!
Yours sincerely.

Chao Chao


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Re: Why my maker get no results?

Carson Holt-2
You can merge datastore indexes that way. You will need to run them separately (i.e. unmodified location and content from what MAKER gave you), and then merge the fasta and gff3 files afterwards.

—Carson


On May 1, 2017, at 7:32 AM, [hidden email] wrote:

Dear sir:
    I' have bben working on genome annotation these days.My process in as below:
    
    1. I split my contigs into 300 parts and deal with them simultaneously to speed up my process. 
    2. I used my splited-genome, protein, ESTs and RNA-seq to make the first alignment( est2genome=1,  AED_threshold=0.2 ). 
    3. Merge the maker.*_.master_datastore_index.log to get all the paths of results.
    4. gff_merge script to merge all the results in different dirs.

    However, there is no results returned. (My genome is about 3GB, but the gff of result is none.)
    index_all.log.all.gff    1KB
    index_all.log.all.maker.proteins.fasta    2837KB
    index_all.log.all.maker.transcripts.fasta    9866KB




    Where can the problems take place?
    Thanks!
Yours sincerely.

Chao Chao

_______________________________________________
maker-devel mailing list
[hidden email]
http://box290.bluehost.com/mailman/listinfo/maker-devel_yandell-lab.org


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[hidden email]
http://box290.bluehost.com/mailman/listinfo/maker-devel_yandell-lab.org
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