maker_functional_gff error

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maker_functional_gff error

Gongyuan Cao
Hi,

I'm running maker_functional_gff and got this error:
Can't use string ("") as a HASH ref while "strict refs" in use at /root/maker/bin/maker_functional_gff line 55, <$IN> line 3.
I've checked the gff file and there are no missing "ID=" tags, what could be the problem?

head of blastpoutput:

lacu_11543-RA A4GSN8 49.643 2099 951 36 1 2026 1 2066 0.0 1724

lacu_11544-RA F4IF36 75.473 1268 273 6 33 1263 29 1295 0.0 1949

lacu_11548-RA O81123 51.316 380 144 10 24 401 15 355 2.29e-119 353

lacu_11549-RA Q9SA32 60.767 339 130 3 328 664 58 395 1.54e-141 421

lacu_11547-RA Q9SLK2 72.493 349 96 0 1 349 1 349 0.0 518

lacu_11558-RA Q9LTV6 76.689 296 69 0 5 300 3 298 2.21e-158 446

lacu_11557-RA Q9C9U5 40.441 272 145 6 866 1134 746 1003 7.55e-50 196

lacu_11552-RA Q96GG9 44.715 246 128 3 58 296 2 246 2.30e-73 229

lacu_11560-RA Q42961 89.375 480 47 2 2 480 4 480 0.0 855

lacu_11561-RA Q42962 91.022 401 36 0 1 401 1 401 0.0 731


head of gff:

##gff-version 3

Linkage_group_5 . contig 1 30484050 . . . ID=Linkage_group_5;Name=Linkage_group_5

Linkage_group_5 maker gene 10601 29761 . + . ID=lacu_11543;Name=lacu_11543;Alias=maker-Linkage_group_5-pred_gff_est2genome-gene-0.188;score=1168;

Linkage_group_5 maker mRNA 10601 29761 6483 + . ID=lacu_11543-RA;Parent=lacu_11543;Name=lacu_11543-RA;Alias=maker-Linkage_group_5-pred_gff_est2genome-gene-0.188-mRNA-1;_AED=0.00;_QI=105|1|1|1|1|1|48|246|2043;_eAED=0.00;score=1168;

Linkage_group_5 maker exon 10601 11011 . + . ID=lacu_11543-RA:exon:0;Parent=lacu_11543-RA;

Linkage_group_5 maker exon 11129 11275 . + . ID=lacu_11543-RA:exon:1;Parent=lacu_11543-RA;

Linkage_group_5 maker exon 11403 11501 . + . ID=lacu_11543-RA:exon:2;Parent=lacu_11543-RA;

Linkage_group_5 maker exon 11835 11963 . + . ID=lacu_11543-RA:exon:3;Parent=lacu_11543-RA;

Linkage_group_5 maker exon 12054 12146 . + . ID=lacu_11543-RA:exon:4;Parent=lacu_11543-RA;

Linkage_group_5 maker exon 12240 12305 . + . ID=lacu_11543-RA:exon:5;Parent=lacu_11543-RA;



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